MATERIALS AND METHODS
A total of 132 patients who would had been undergone cardiac angiogram at Cardiac Medical Unit of Yangon General Hospital during the period of May 2016 and June 2017 participated in the hospital-based comparative study. After getting the written informed consent, the patients were instructed for overnight fasting. After that, their fasting blood sample was taken. The data of the coronary angiogram of the patients was also recorded and the presence and absence of CAD were categorized into two groups in data analysis. The blood pressure, weight and height were taken from patient medical record. The BMI was calculated from patient’s weight and height. The body fat percent was calculated by the formula.18 The presence of risk factors such as diabetes mellitus and hypertension were found out by questionnaires. There were no exclusion criteria in selection of patient. The patients who were currently taking lipid lowering therapy were also included in current study.
The fasting sera were then analyzed for HDL2-C and HDL3-C levels by single pre-cipitation procedure with dextran sulphate, heparin sodium and MnCl2 solution followed by enzymatic colorimetric assay for cho-lesterol.19 The serum total cholesterol (TC) and triglyceride (TG) levels were also deter-mined by enzymatic colorimetric method.20, 21 Serum low-density lipoprotein cholesterol (LDL-C) was determined by using formula modified by McNamara, et al (1990).22
Data entry and analysis were done by using Statistical Package for Social Sciences, SPSS 21 version. Summary statistics of the range, the mean, the median, the standard deviation, interquartile range and the percentiles were calculated for HDL-C, HDL2-C, HDL3-C, HDL2-C/HDL3-C, TC and TG. Due to the skewness of the data, comparisons of the HDL-C, HDL2-C, HDL3-C, HDL2-C/HDL3-C, TC and TG in CAD and control were done by Mann-Whitney U test. The associations between HDL-C, HDL2-C, HDL3-C, HDL2-C/HDL3-C and CAD were calculated by Pearson Chi-square (χ2) test. Correlations of HDL-C, HDL2-C, HDL3-C, HDL2-C/HDL3-C, TC and TG were cal-culated by Spearman correlation coefficient (ρ). The stronger significant level was set at P<0.05.