Myanmar Health Sciences Research Journal

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MATERIALS AND METHODS

 

Reagents and instruments

Analytical grade standard of calcium (Ca), copper (Cu), iron (Fe), magnesium (Mg), manganese (Mn), potassium (K), sodium (Na) and zinc (Zn), 70% nitric acid (HNO3) and  69% hydrochloric acid (HCl), double deionized water (DDW) were used. Atomic Absorption Spectrophotometer (AAS), AA 6650 and muffle furnace (LEF 1035) were used for this study.

Plant authenticity

Fennel seeds and ginger were identified
and confirmed for its specific botanical name by competent taxonomist
according to The Flora of Ceylon.14

Sample collection

Fennel seeds were collected from the farms of Myin Gyan (MGN) and  Pyin Oo Lwin (POL) townships. Rhizomes of ginger was collected from Mandalay (Mdy) and POL townships, respectively.

Phytochemical test

Types of compounds present in these samples were tested according to the methods of Harborne, 1998.15

Sample preparation for AAS

Cleaned dried each sample was crushed, powdered and homogenized, using mortar and pestle, and then kept in sampling bags for further analysis.

Digestion of samples

Specified weight (2.5 g) of each powder sample was placed into crucible for heating in an oven at 110ºC for 2 hours to remove moisture. These dried samples were placed in muffle furnace at 550ºC for 4 hours until the sample became totally ashes. After that,
5 ml of 6 M HNO3 were added to each ash sample to dissolve and digest the contents. Then, each solution was filtered and diluted with DDW up to 50 ml in volumetric flask.
16 Standard solutions of each metal were separately prepared from their respective concentration of 1000 mg/ml stock solutions, for the standard calibration curve. Reagent blank determination was used to correct the instrument readings.7

Statistical analysis

Data were analyzed by using Microsoft Office Excel v. 2007. Results were presented as mean±SE.

 

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